Firefly Luc mRNA (unmodified)
Croyez Firefly Luciferase mRNA expresses luciferase protein derived from the firefly Photinus pyralis. This enzyme facilitates ATP-dependent D-luciferin oxidation, resulting in the production of oxyluciferin—a light-emitting compound upon returning to its ground state. Widely used as a bioluminescent reporter, our mRNA, supplied at 1 mg/ ml, features a Cap 1 structure, and poly A tail. The Cap 1 structure ensures efficient translation, while poly A tail minimize host cell immune response and enhance mRNA stability. Ideal for gene expression assays, cell viability studies, and in vivo imaging, Croyez Firefly Luciferase mRNA is designed for effective and versatile applications in gene regulation research.
mRNA length:
2257 nt
Base composition:
Unmodified
Concentration:
1.0 mg/ mL
Cap modification:
Cap 1 structure
Poly A tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
2257 nt
Base composition:
Unmodified
Concentration:
1.0 mg/ mL
Cap modification:
Cap 1 structure
Poly A tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
Figure 1.293T cells (0.8 × 10⁵ cells per well, 24-well plate) were transfected with 1 µg of Firefly Luc mRNA using the EndoSafe mRNA Transfection Kit (C15053-K01).
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Marker: Croyez High Range RNA ladder (cat no: CR0005)
Figure 3. Capillary Electrophoresis
Firefly Luciferase mRNA was analyzed by capillary electrophoresis.
